目的 分离纯化驱虫斑鸠菊中紫铆素、紫铆花素,初步探讨这两种化合物对人A375黑素瘤细胞增殖及黑素合成作用的影响。方法 采用硅胶、Sephadex LH-20反复柱层析分离纯化紫铆素、紫铆花素;分别采用MTT法、酶学法及NaOH法测定这两种化合物对人A375黑素瘤细胞的增殖、酪氨酸酶活性及黑素合成的影响。结果 分离得到化合物紫铆素、紫铆花素,在0.50~10.00 μg·mL-1内紫铆素、紫铆花素促进A375细胞增殖,且紫铆花素促增殖作用强于紫铆素(P<0.05);在0.10~1.00 μg·mL-1内两种化合物均上调酪氨酸酶活性;在0.50~5.00 μg·mL-1内,紫铆花素促进黑素合成,紫铆素抑制黑素合成。结论 紫铆素、紫铆花素可能是驱虫斑鸠菊的药效成分;紫铆花素促进A375细胞的增殖,且使 A375细胞黑素合成增加,紫铆素轻度抑制黑素合成。
Abstract
To purify butin and butein from the Vernonia anthelmintica Willd,and preliminary study their effects on proliferation and melanogenesis of A375 human melanoma cells. METHODS Selica gel and Sephadex LH-20 column chromatography methods were used to separate butin and butein; their effects on proliferation and melanogenesis of A375 human melanoma cells were studied by MTT method,enzyme method and NaOH method. RESULTS Butin and butein were isolated and identified. In the concentration range of 0.50-10.00 μg·mL-1,butin and butein enhanced the proliferation of A375 human melanoma cells,and the effect of butein was stronger than butin(P<0.05); in the concentration range of 0.10-1.00 μg·mL-1,both butin and butein increased the activity of tyrosinase; in the concentration range of 0.50-5.00 μg·mL-1,butein stimulated melanogenesis,but butin inhibited melanogenesis. CONCLUSION Butin and butein may be the active components of Vernonia anthelmintica Willd; butein promotes the proliferation and stimulates the melanogenesis of A375 human melanoma cells,however,butin mildly inhibites the melanogenesis.
关键词
驱虫斑鸠菊 /
紫铆素 /
紫铆花素 /
分离纯化 /
人A375黑素瘤细胞 /
黑素合成
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Key words
Vernonia anthelmintica Willd /
butin /
butein /
purification /
A375 human melanoma cells /
melanogenesis
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中图分类号：
R282
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参考文献
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脚注
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基金
国家自然科学基金资助项目(30960506,81160524);宁夏自然科学基金(NZ11212);宁夏医科大学重点项目(XM2011017)
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